BRAIN HEART INFUSION BROTH

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store tubed or bottled medium in the dark and below 20°C.	Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Upstream tips
-Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store tubed or bottled medium in the dark and below 20°C.
Protocol tips
Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Publication protocol

-The P. aeruginosa overnight culture was suspended in Brain Heart Infusion (BHI, Oxoid, UK) supplemented with 4% glucose (BHI-g) to an optical density at 600 nm (OD600) of 0.5. The culture was diluted 1009 in the microtiter plate. Following the incubation carried out at
37 °C for 24 h, the microtiter plate wells were washed three times with water, and the biofilm fixed by air drying. The adherent biofilm layer was stained with crystal violet (2% solution) and dried; subsequently, the dye was redissolved with 150 lL of ethanol and measured at OD595. Each strain was tested three times.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for BRAIN HEART INFUSION BROTH below.

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