BRAIN HEART INFUSION BROTH

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store tubed or bottled medium in the dark and below 20°C.	-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Upstream tips
-Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store tubed or bottled medium in the dark and below 20°C.
Protocol tips
-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Publication protocol

Before use in the experiments, strains were activated in BHI broth at 37 °C for 18–24h, reaching a concentration of approximately 108 cfu.mL−1.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for BRAIN HEART INFUSION BROTH below.

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