BHI - Brain Heart Infusion

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store between 15-25°C.	- With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended.

Upstream tips
-Store between 15-25°C.
Protocol tips
- With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended.

Publication protocol

All the pathogenic bacteria were cultured in brain heart infusion (BHI) broth (HiMedia) and Candida spp. was cultured in Sabouroud dextrose broth (HiMedia) at 37°C under aerobic conditions. All the cultures were stored at −80°C in broth supplemented with 20% glycerol.

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Manufacturer protocol

Download the product protocol from HiMEDIA for BHI - Brain Heart Infusion below.

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