EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Ensure that all buffers are in clear solution. Shake or vortex if these buffers precipitate.	-When processing spin columns, always cap spin columns before placing them in the microcentrifuge. -The conditions of crosslinked DNA shearing can be optimized based on cells and sonicator equipment. If desired, remove 5 µl of sonicated cell lysate for agarose gel analysis. The length of sheared DNA should be between 200-1000 bp.

Upstream tips
-Ensure that all buffers are in clear solution. Shake or vortex if these buffers precipitate.
Protocol tips
-When processing spin columns, always cap spin columns before placing them in the microcentrifuge. -The conditions of crosslinked DNA shearing can be optimized based on cells and sonicator equipment. If desired, remove 5 µl of sonicated cell lysate for agarose gel analysis. The length of sheared DNA should be between 200-1000 bp.

Publication protocol

Molecular events in the kidneys of LPS-treated mice were monitored using a chromatin immunoprecipitation (ChIP) assay to immunopurify soluble chromatinized H3K4 me3 sequences using specific antibodies and the EpiQuik ChIP kit (EPIGENTEK, Brooklyn, NY, USA). The antibodies used included normal mouse IgG as the negative control, anti-RNA polymerase II as the positive control, and anti-H3K4 me3 (EPIGENTEK, Brooklyn, NY, USA). The DNA concentration was quantified using a NanoDrop UV spectrometer (NanoDrop Technologies, Wilmington, DE, USA). A SYBR green assay (LightCycler 480 SYBR Green I Master; Roche, Mannheim, Germany) was performed using the 7300HT Fast Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA) using primers for mouse cathepsin L (EpiTect ChIP qPCR Assays, QIAGEN).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ChIP Mouse - Kidney using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit from Epigentek.

Manufacturer protocol

Download the product protocol from Epigentek for EpiQuik Chromatin Immunoprecipitation (ChIP) Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ChIP Mouse - Kidney using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit from Epigentek. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.