Cytoselect™ Cell Viability and Cytotoxicity Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add the MTT Reagent to each well at a 1:10 ratio. For example, add 10 µL/well for a 96-well plate or 25 µL/well for a 24-well plate. Incubate the wells 2-4 hours or overnight at 37°C. Monitor the cells occasionally with an inverted microscope for the presence of a purple precipitate

Protocol tips
Add the MTT Reagent to each well at a 1:10 ratio. For example, add 10 µL/well for a 96-well plate or 25 µL/well for a 24-well plate. Incubate the wells 2-4 hours or overnight at 37°C. Monitor the cells occasionally with an inverted microscope for the presence of a purple precipitate

Publication protocol

Cells were plated in quadruplicates in 96-well plates and incubated at 37 °C with 5% CO2 overnight for attachment and growth. Approximately 60% confluent cells were treated with different doses of ASA dissolved in DMSO (Sigma-Aldrich) or DMSO alone (control) for 72 h. Cellular viability was measured using crystal violet-based cell viability and cytotoxicity assay (CytoSelectTM Cell Viability and Cytotoxicity Assay kits, Cell Biolabs, San Diego, CA, USA) as described earlier by Kambhampati et al18 and Trypan blue exclusion assay (Life Technologies, Grand Island, NY, USA).



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Manufacturer protocol

Download the product protocol from Cell Biolabs for Cytoselect™ Cell Viability and Cytotoxicity Assay below.

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