Chromatin Immunoprecipitation (ChIP) Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Just because an antibody works well in a Western blot does not always indicate it will perform well in chromatin immunoprecipitation. Unlike a Western blot that detects proteins that have been denatured, a ChIP antibody must recognize the target protein in its native state. Use ChIP-validated antibody. -Use 2-10 μg of your ChIP antibody depending on the abundance of your protein target.

Protocol tips
-Just because an antibody works well in a Western blot does not always indicate it will perform well in chromatin immunoprecipitation. Unlike a Western blot that detects proteins that have been denatured, a ChIP antibody must recognize the target protein in its native state. Use ChIP-validated antibody. -Use 2-10 μg of your ChIP antibody depending on the abundance of your protein target.

Publication protocol

According to the manufacturer’s instruction, ChIP assays were performed using an EZ-ChIP kit (Millipore, USA). For immunoprecipitation, 5 µg of rabbit antibody anti-Rad21 (ab992, Abcam), anti-SMC3 (ab9263, Abcam), anti-Sin3α (ab3479, Abcam), anti-EZH2 (#5246, Cell Signaling Technology), H3 trimethyl Lys 27 antibody (Millipore, USA), or normal rabbit IgG (Millipore) was used, and DNA quantification was performed by qPCR with SYBR Green Mix (Takara).

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Manufacturer protocol

Download the product protocol from Merck Millipore for Chromatin Immunoprecipitation (ChIP) Assay Kit below.

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