|Prepare a mix by adding 2 µL of 1 M calcein-AM stock solution and 3 µL of 1.5 M PI solution to 1 mL of Dulbecco’s modified Eagle’s medium supplemented with 2 mM of l-glutamine, 100 U/mL of penicillin and 100 µg/mL of streptomycin.|
|Mix 100 µl of the staining solution with 200 µl of the cell suspension, and incubate the mixture for 15 minutes at 37C|
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