ToxCount™ Cell Viability Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
All working solutions can be prepared with either PBSg(pbs glucose) or serum free medium.	Add 50 µl of dye mixture to each well. Incubate cells at 37˚C for 30 minutes

Upstream tips
All working solutions can be prepared with either PBSg(pbs glucose) or serum free medium.
Protocol tips
Add 50 µl of dye mixture to each well. Incubate cells at 37˚C for 30 minutes

Publication protocol

Cell viability was measured using ToxCount assays per the manufacturer's recommendations (Active Motif). GSCs were plated in laminin coated 96-well plates at a density of 2,000 cells per well, allowed to attach overnight, and treated with either DMSO or DSF, ranging from 0.01 – 10 μM, for 96 hours. For investigating the effect of metal ions on cell viability, GSCs were treated with DSF combined with either copper sulfate or zinc gluconate (at a 1:1 molar ratio). Prior to taking proliferation measurements, ToxCount reagents were incubated with GSCs for 30 minutes at 37 °C. The conversion of calcein AM to green fluorescent calcein in viable cells was measured using the IsoCyte (BlueShift Biotechnologies) with a 510/540 nm band pass filter. Percentage cell viability is reported as the mean and SD (of six replicate measurements) relative to DMSO control.

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Manufacturer protocol

Download the product protocol from Active Motif for ToxCount™ Cell Viability Assay below.

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