ChromaFlash Chromatin Extraction Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Spin the solution down to the bottom prior to use. - Check if any buffers contain salt precipitates before use. If so, shake the buffer until the salts are re-dissolved.	-For optimal preparation, the input amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues. -Avoid multiple freeze/thaw cycles.

Upstream tips
- Spin the solution down to the bottom prior to use. - Check if any buffers contain salt precipitates before use. If so, shake the buffer until the salts are re-dissolved.
Protocol tips
-For optimal preparation, the input amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues. -Avoid multiple freeze/thaw cycles.

Publication protocol

Chromatin preparation in whole bone homogenate was carried out using ChromaFlash Chromatin Extraction Kit (Base Catalog # P-2001 Epigentek, Farmingdale, NY, USA) as described by the manufacturer’s instructions. Chromatin immunoprecipitation assay was performed as per previously described41. Briefly, the immunoprecipitated DNA (through anti-H3K27ac antibody) was amplified by PCR reaction using GoTaq® Hot Start Green Master Mix (Promega, USA) and PCR was performed by using S1000™ Thermal Cycler-BIORAD. Primers were designed for the targeted gene promoter.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ChIP Mouse - Osteoblasts using ChromaFlash Chromatin Extraction Kit from Epigentek.

Manufacturer protocol

Download the product protocol from Epigentek for ChromaFlash Chromatin Extraction Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ChIP Mouse - Osteoblasts using ChromaFlash Chromatin Extraction Kit from Epigentek. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.