ChIP-IT® Express Chromatin Immunoprecipitation Kits

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Do not re-freeze the Protein G magnetic beads, the beads should be stored at 4C.	-Always use ChIP validated antibody. -Optimal use of 1-3 ug of antibody.	-The diluted Proteinase K stop solution can not be stored.

Upstream tips
-Do not re-freeze the Protein G magnetic beads, the beads should be stored at 4C.
Protocol tips
-Always use ChIP validated antibody. -Optimal use of 1-3 ug of antibody.
Downstream tips
-The diluted Proteinase K stop solution can not be stored.

Publication protocol

Chromatin immunoprecipitation (ChIP) assay was performed using the ChIP-ITTM kit (Active Motif) according to the manufacturer’s instructions. Chromatin was immunoprecipitated with 2 μg of either rabbit LXR antibody (Santa Cruz Biotechnology Inc) or negative control IgG. DNA was analyzed by PCR to amplify regions containing the putative LXR binding sites on rat ABCA1 promoter by using primers forward 5′-GCTTTCTGCTGAGTGACTGAACTAC-3′ and reverse 5′-GAATTACTGCTTTTTGCCGCG-3′ as described previously.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ChIP Rat - INS-1 using ChIP-IT® Express Chromatin Immunoprecipitation Kits from Active Motif.

Manufacturer protocol

Download the product protocol from Active Motif for ChIP-IT® Express Chromatin Immunoprecipitation Kits below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ChIP Rat - INS-1 using ChIP-IT® Express Chromatin Immunoprecipitation Kits from Active Motif. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.