EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Ensure that all buffers are in clear solution. Shake or vortex if these buffers precipitate.	-When processing spin columns, always cap spin columns before placing them in the microcentrifuge. -The conditions of crosslinked DNA shearing can be optimized based on cells and sonicator equipment. If desired, remove 5 µl of sonicated cell lysate for agarose gel analysis. The length of sheared DNA should be between 200-1000 bp.

Upstream tips
-Ensure that all buffers are in clear solution. Shake or vortex if these buffers precipitate.
Protocol tips
-When processing spin columns, always cap spin columns before placing them in the microcentrifuge. -The conditions of crosslinked DNA shearing can be optimized based on cells and sonicator equipment. If desired, remove 5 µl of sonicated cell lysate for agarose gel analysis. The length of sheared DNA should be between 200-1000 bp.

Publication protocol

The ChIP assays were performed using an EpiQuikTM chromatin immunoprecipitation kit from Epigentek Group Inc. (Brooklyn, NY) and according to the published methods.

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Manufacturer protocol

Download the product protocol from Epigentek for EpiQuik Chromatin Immunoprecipitation (ChIP) Kit below.

Download manufacturer protocol

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