ChromaFlash High-Sensitivity ChIP Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Ensure that all buffers are in clear solution. Shake or vortex if these buffers precipitate.	-When processing spin columns, always cap spin columns before placing them in the microcentrifuge. -The conditions of crosslinked DNA shearing can be optimized based on cells and sonicator equipment. If desired, remove 5 µl of sonicated cell lysate for agarose gel analysis. The length of sheared DNA should be between 200-1000 bp.

Upstream tips
-Ensure that all buffers are in clear solution. Shake or vortex if these buffers precipitate.
Protocol tips
-When processing spin columns, always cap spin columns before placing them in the microcentrifuge. -The conditions of crosslinked DNA shearing can be optimized based on cells and sonicator equipment. If desired, remove 5 µl of sonicated cell lysate for agarose gel analysis. The length of sheared DNA should be between 200-1000 bp.

Publication protocol

ChIP assays were conducted using the ChromaFlash™ High-Sensitivity ChIP Kit (EPIGENTEK, Base Catalog # P-2027). All the steps of experiment followed the assay protocol. Rats' DRGs were homogenized and cross-linked with 1% formaldehyde for 10 min at room temperature. Add 0.25 M glycine to terminated reactions and centrifuge at 5000 rpm for 5 min at 4 °C. Carefully remove supernatant and resuspend the chromatin lysate by vortexing and sheared chromatin using probe-based sonication. For immunoprecipitation, the sample used the Histone H3 (Rabbit, Cell Signaling Technology) binding to the Assay Strip Wells.

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Manufacturer protocol

Download the product protocol from Epigentek for ChromaFlash High-Sensitivity ChIP Kit below.

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