Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
Store entire kit at room temperature. After breaking foil on microarray pouch, store microarray slides at room temperature (in the dark) under a vacuum dessicator or nitrogen purge box. Do not store microarray slides in open air after breaking foil. |
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Upstream tips |
Store entire kit at room temperature. After breaking foil on microarray pouch, store microarray slides at room temperature (in the dark) under a vacuum dessicator or nitrogen purge box. Do not store microarray slides in open air after breaking foil. |
Publication protocol
RNAs obtained from cells were amplified and labeled starting from 150 ng and using the Low Input Quick Amp Labeling Kit, one-color (Agilent Technologies) as described by the proprietary protocol. Labeled cRNA was purified with miRNeasy Mini Kit (QIAGEN) and quantified using NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific). On average, cRNA yield was about 10 μg and the specific activity was of 35 pmol Cy3 per μg cRNA.
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Manufacturer protocol
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