Anti-acetyl-Histone H3 (Lys9) Antibody

Protocol tips

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	The ChIP assay was performed using the imprint chromatin immunoprecipitation kit (CHP1, Sigma-Aldrich) following the manufacturer's instructions. The sonicated DNA was incubated with anti-IgG (Millipore, Milford, MA, USA) as a negative control. The purified DNA was amplified using specifically designed real-time ChIP PCR primers spanning the region −300 to +1000 of LY6K.

Protocol tips
The ChIP assay was performed using the imprint chromatin immunoprecipitation kit (CHP1, Sigma-Aldrich) following the manufacturer's instructions. The sonicated DNA was incubated with anti-IgG (Millipore, Milford, MA, USA) as a negative control. The purified DNA was amplified using specifically designed real-time ChIP PCR primers spanning the region −300 to +1000 of LY6K.

Publication protocol

The ChIP assay was performed using the imprint chromatin immunoprecipitation kit (CHP1, Sigma-Aldrich) following the manufacturer's instructions. The sonicated DNA was incubated with anti-IgG (Millipore, Milford, MA, USA) as a negative control, anti- H3K9ac (Millipore), anti-H3K4me (Millipore), anti-H3K9me3 (Abcam, Cambridge, MA, USA) and anti-H3K27me3 (Millipore) at 37°C for 90 min. The purified DNA was amplified using specifically designed real-time ChIP PCR primers spanning the region −300 to +1000 of LY6K.

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Manufacturer protocol

Download the product protocol from Merck Millipore for Anti-acetyl-Histone H3 (Lys9) Antibody below.

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