LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Protocol tips

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	Add 8 µM ethidium homodimer and 2 µM calcein acetoxymethyl in PBS and incubate for 30~45 min at room temperature

Protocol tips
Add 8 µM ethidium homodimer and 2 µM calcein acetoxymethyl in PBS and incubate for 30~45 min at room temperature

Publication protocol

A two-color fluorescent cell cytotoxicity assay was used to confirm the results obtained from the colorimetric cell viability assay. A LIVE/DEAD viability/cytotoxicity kit was purchased from Molecular Probes (Eugene, OR, USA). After a 24 hr tissue adhesive exposure, cells were incubated with a mixture of 8 µM ethidium homodimer and 2 µM calcein acetoxymethyl in PBS for 30~45 min at room temperature. Images were collected using a Zeiss LSM-700 confocal microscopy system (Thornwood, NY, USA). The number of viable (green) and non-viable (red) cells were counted manually from the images.

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