Live and Dead Cell Assay (Abcam)

Protocol tips

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	Add 4 μM of calcein-acetomethoxy (calcein AM-5 μL) and 2 mM of ethidium-bromide (20 μL). Incubate this mixture for 30–45 min at room temperature in the dark

Protocol tips
Add 4 μM of calcein-acetomethoxy (calcein AM-5 μL) and 2 mM of ethidium-bromide (20 μL). Incubate this mixture for 30–45 min at room temperature in the dark

Publication protocol

Cell-drug interaction was also evaluated using cell viability assay (live-dead staining kit) over the period of 21 days. From the stock solution, 4 μM of calcein-acetomethoxy (calcein AM-5 μL) and 2 mM of ethidium-bromide (20 μL) were diluted in 10 mL PBS (tissue culture grade). 20–50 μL of working solution was added to each well and plates were incubated for 30–45 min and labeled cells were observed under Nikon fluorescent microscope as per general protocol [23].

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