Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Add protease inhibitors to all lysis solutions before use.	-Use 2-4 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Upstream tips
-Add protease inhibitors to all lysis solutions before use.
Protocol tips
-Use 2-4 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Publication protocol

The ChIP assay was performed using the imprint chromatin immunoprecipitation kit (CHP1, Sigma-Aldrich) following the manufacturer's instructions. The sonicated DNA was incubated with anti-IgG (Millipore, Milford, MA, USA) as a negative control, anti- H3K9ac (Millipore), anti-H3K4me (Millipore), anti-H3K9me3 (Abcam, Cambridge, MA, USA) and anti-H3K27me3 (Millipore) at 37°C for 90 min. The purified DNA was amplified using specifically designed real-time ChIP PCR primers spanning the region −300 to +1000 of LY6K.

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Manufacturer protocol

Download the product protocol from Abcam for Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) below.

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