Histone H3K36me3 antibody (mAb)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-5 - 10 µg per ChIP -Avoid over fixation. -purchase expensive, "hing end" sonicator to get reproducible shearing. -Avoid repeated freeze/thaw cycles. -Keep all reagents on ice when not in storage.

Protocol tips
-5 - 10 µg per ChIP -Avoid over fixation. -purchase expensive, "hing end" sonicator to get reproducible shearing. -Avoid repeated freeze/thaw cycles. -Keep all reagents on ice when not in storage.

Publication protocol

ChIP-Seq data were analyzed using CAM (66). The aggregation plots of both vector-IRF1 sample and Lv1-F, IRF1 samples over promoter regions were generated in 10-bp resolution. Multiple key quality control measurements such as sequencing coverage and nucleosome organization profiles were generated and compared with a set of 268 historical MNase-Seq datasets in human and mouse that serve as a reference.

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Papers

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Manufacturer protocol

Download the product protocol from Active Motif for Histone H3K36me3 antibody (mAb) below.

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