Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Add protease inhibitors to all lysis solutions before use.	-Use 2-25 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Upstream tips
-Add protease inhibitors to all lysis solutions before use.
Protocol tips
-Use 2-25 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Publication protocol

ChIP-exo was performed as previously described6,20 with chromatin extracted from 50 million cells, ProteinG MagSepharose resin (GE Healthcare), and 5 ug of antibody directed against RNA polymerase II (Santa Cruz sc899), H2A.Z (EMD Millipore 07-594), H3K4me3 (Abcam ab8580), H3K4me2 (Abcam ab7766), H3K4me1 (Abcam ab8895), H3K27ac (Abcam ab4729), H4K9Ac (Abcam ab4441), H3K27me3 (EMD Millipore 07-449), H4K20me1 (Abcam ab9051), and CTCF (EMD Millipore 07-729). Two biological replicates were prepared for each ChIP target. To enable future comparisons to drug treatment experiments, cells were treated with vehicle (final 0.03% DMSO (dimethyl sulfoxide)) for 4 h prior to harvest. Libraries were sequenced using an Illumina NextSeq500 sequencer as single-end reads 50 or 75 nucleotides in length.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ChIP Anti-bodies H4K20me1 using Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ChIP Anti-bodies H4K20me1 using Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.