Recombinant Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Add protease inhibitors to all lysis solutions before use.	-Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Upstream tips
-Add protease inhibitors to all lysis solutions before use.
Protocol tips
-Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Publication protocol

Chromatin immunoprecipitation assays were performed according to the manufacturer's protocol (ChIP Kit Magnetic One-Step; Abcam, Cambridge, UK). The GATA3 antibody purchased from Abcam (cat no. ab199428; dilution, 1:25), were used for the chromatin immunoprecipitation assays. The GATA3 binding region was amplified using a primer set as follows: Forward, 5′-CCAAAGTAAGTC-3′ and reverse, 5′-CTTCCCTCCAGGG-3′.

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Manufacturer protocol

Download the product protocol from Abcam for Recombinant Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428) below.

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