GATA-3 Antibody (HG3-31): sc-268

Protocol tips

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	-Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases.

Protocol tips
-Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases.

Publication protocol

ChIP assays were performed using anti-Gata3 (mixed 1:1, Santa Cruz Biotechnology; HG3-31, R&D Systems; 634913), anti-JunB (Santa Cruz Biotechnology; N-17), anti-Ets (Santa Cruz Biotechnology; C-20), anti-trimethyl histone H3-K4 (Millipore), anti-acetyl histone H3-K9 (Millipore), anti-RNA polymerase II (Abcam), anti-RNA polymerase II phospho-CTD Ser-5 (Abcam), anti-RNA polymerase II phospho-CTD Ser-2 (Active Motif), and anti-FLAG (M2) antibodies as described previously (36). The quantitative-PCR analyses were performed on an ABI prism 7500 real-time PCR machine with probes from the Roche Diagnostics Universal Probe Library System. The specific primers and TaqMan probes used in this study were described previously.

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