Sall4 Antibody (EE-30): sc-101147

Protocol tips

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	-Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases.

Protocol tips
-Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases.

Publication protocol

An EZ‐ChIP™ Assay Kit (Cat#17–371; Millipore) was used to perform quantitative chromatin immunoprecipitation (qChIP) assays according to the manufacturer's protocol. In brief, cells were treated with 37% formaldehyde to crosslink proteins, and then the reaction was terminated with 0.125 M glycine. After sonication, chromatin–protein complexes were immunoprecipitated with 5 μg of anti‐Sall4 antibody (sc‐101147; Santa Cruz). Regions of interest were amplified in triplicate from precipitated samples by real‐time PCR, and the amount of precipitated DNA was calculated as a percentage of the input sample.

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