Histone H2A.X antibody (pAb)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Avoid over fixation. -purchase expensive, "hing end" sonicator to get reproducible shearing. -Avoid repeated freeze/thaw cycles. -Keep all reagents on ice when not in storage.

Protocol tips
-Avoid over fixation. -purchase expensive, "hing end" sonicator to get reproducible shearing. -Avoid repeated freeze/thaw cycles. -Keep all reagents on ice when not in storage.

Publication protocol

The cells were initially plated at a density of 5 × 105 cells in a 10-cm dish. One day after seeding, the cells were washed twice with prewarmed PBS at 37°C and incubated with or without 100 ng/ml nocodazole-containing medium. Eighteen hours later, the media were removed and the cells were washed twice with prewarmed PBS. Mitotic arrested cells were then harvested and used either for western blotting or ChIP.

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Papers

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Manufacturer protocol

Download the product protocol from Active Motif for Histone H2A.X antibody (pAb) below.

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Videos

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