Recombinant Anti-PARP1 antibody [E102] (ab32138)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Add protease inhibitors to all lysis solutions before use.	-Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Upstream tips
-Add protease inhibitors to all lysis solutions before use.
Protocol tips
-Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Publication protocol

The cells were initially plated at a density of 5 × 105 cells in a 10-cm dish. One day after seeding, the cells were washed twice with prewarmed PBS at 37°C and incubated with or without 100 ng/ml nocodazole-containing medium. Eighteen hours later, the media were removed and the cells were washed twice with prewarmed PBS. Mitotic arrested cells were then harvested and used either for western blotting or ChIP.

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Papers

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Manufacturer protocol

Download the product protocol from Abcam for Recombinant Anti-PARP1 antibody [E102] (ab32138) below.

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