QIAGEN Large-Construct Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Starting material <500 ml culture volume

Protocol tips
Starting material <500 ml culture volume

Publication protocol

The construct was transformed into DH10Bac E. coli (Invitrogen) where the Igκ-T2 insert was spontaneously transposed into bacmid. The resultant recombinant bacmid designated BacIgκ-T2 was purified by Large-Construct Kit (Qiagen, Hilden, Germany) and transfected into Sf9 cells using Cellfectin reagents (Invitrogen).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Plasmid Isolation DH10Bac (Bacmid) using QIAGEN Large-Construct Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for QIAGEN Large-Construct Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Plasmid Isolation DH10Bac (Bacmid) using QIAGEN Large-Construct Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.