Nucleic Acid Purification

Protocol tips

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	Starting material from 1–5 mL cultures

Protocol tips
Starting material from 1–5 mL cultures

Publication protocol

The constructed plasmid was designated as pFastBac1-gE2. DH10Bac competent cells were transformed with pFastBac1-gE2, and the single colonies were inoculated into Luria-Bertani (LB) medium. Recombinant plasmid was extracted from competent cells using a plasmid DNA extract kit according to the product instructions (Tiangen, China) and confirmed by PCR.

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Manufacturer protocol

Download the product protocol from Tiangen for Nucleic Acid Purification below.

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