GenElute™ Plasmid Miniprep Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Starting material 1-5 mL of cultures

Protocol tips
Starting material 1-5 mL of cultures

Publication protocol

Transformation of strain IMX031 with the URA3-bearing mhpF-expression plasmid pUDE43 (Guadalupe Medina et al., 2010) yielded the prototrophic, Gpd– mhpF-expressing strain IMZ160. Plasmid(s) were isolated from S. cerevisiae IMZ333 with the Sigma GenElute plasmid miniprep kit (Sigma-Aldrich Chemie Gmbh, Munich, Germany) according to manufacturer's instructions.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for GenElute™ Plasmid Miniprep Kit below.

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