GeneJET Plasmid Miniprep Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Starting material 1-5 mL bacterial cultures. If more than 5 mL of such a culture are processed, the GeneJET spin column capacity (20 Hg of dsDNA) will be exceeded and no increase in plasmid yield will be obtained.

Protocol tips
Starting material 1-5 mL bacterial cultures. If more than 5 mL of such a culture are processed, the GeneJET spin column capacity (20 Hg of dsDNA) will be exceeded and no increase in plasmid yield will be obtained.

Publication protocol

The plasmid was extracted using the GeneJet plasmid miniprep kit (Fermentas) with 4 mg/ml lysozyme (Sigma, USA) added to the resuspension buffer, in which resuspended pellets were incubated for 30 min at 37°C.

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for GeneJET Plasmid Miniprep Kit below.

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Videos

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