LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy & quantitative assays

Protocol tips

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	Add 10 µL of the dye mixture for each mL of the bacterial suspension. Mix thoroughly and incubate at room temperature in the dark for 15 minutes

Protocol tips
Add 10 µL of the dye mixture for each mL of the bacterial suspension. Mix thoroughly and incubate at room temperature in the dark for 15 minutes

Publication protocol

LIVE/DEAD BacLight assay kit was purchased from Invitrogen and the assay was performed per manufacturer’s instructions. Briefly, a working solution was prepared by adding 30 µL SYTO 9 stock solution (5 mM) and 30 µl propidium iodide (20 mM) into 1 ml filter-sterilized dH2O. The working solution (10 µl) was pipetted into each well and mixed thoroughly by pipetting up and down. The plate was incubated at room temperature in the dark for 15 minutes. With the excitation wavelength at 485 nm, the fluorescence intensities at 535 nm (green emission) and 635 nm (red emission) were measured using HTS 7000 Plus Bio Assay Reader (Perkin Elmer Inc.) and the ratio of green fluorescence to red fluorescence was calculated.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy & quantitative assays below.

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