Live and Dead Cell Assay (Abcam)

Live / Dead assay bacteria - Staphylococcus aureus

Experiment

Live / Dead assay bacteria - Staphylococcus aureus

Product

Live and Dead Cell Assay (Abcam) from Abcam

Manufacturer

Abcam

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 500 μL of 1X Live and Dead Dye to cells. Incubate 10 min at room temperature

Protocol tips
Add 500 μL of 1X Live and Dead Dye to cells. Incubate 10 min at room temperature

Publication protocol

In addition, the cell viability of various specimens was analyzed by a Live/Dead Cell kit (ab115347, Abcam, Cambridge, UK) as described previously.27 The cell seeding procedures were similar to those of the cell spreading assay. After a 24-h co-culture, cells attached on the specimens were stained with 500 μL of combination dye for 10 min, and were then detected by the CLSM (Leica TCS SP8). The viable cells with esterase activity appeared green, whereas dead cells with compromised plasma membranes appeared red, as described in the manufacturer’s protocol

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Discussion

5 years ago

Author: M. Daecher Germany

Live/dead assay Bacteria

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay bacteria - Staphylococcus aureus using Live and Dead Cell Assay (Abcam) from Abcam.

Paper title

Cytocompatibility with osteogenic cells and enhanced anti-infection potential of quaternized chitosan-loaded titania nanotubes

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Manufacturer protocol

Download the product protocol from Abcam for Live and Dead Cell Assay (Abcam) below.

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