Plasmid Miniprep Kit I, peqGOLD

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Starting Material 5 ml

Protocol tips
Starting Material 5 ml

Publication protocol

Plasmid DNA and genomic DNA were isolated using peqGOLD Plasmid Miniprep Kit I and a peqGOLD bacterial DNA kit (Peqlab Biotechnologie GmbH, Erlangen, Germany), respectively. To improve lysis of S. aureus and B. subtilis, the cells were first incubated for 15 min at 37°C in solution I supplemented with 100 μg/ml lysostaphin (Sigma-Aldrich, St. Louis, MO) and 2 mg/ml lysozyme (Roche Diagnostics, Rotkreuz, Switzerland), respectively.

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Manufacturer protocol

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