Plasmid Mini

Plasmid Isolation Corynebacterium diphtheriae

Experiment

Plasmid Isolation Corynebacterium diphtheriae

Product

Plasmid Mini from A&A Biotechnology

Manufacturer

A&A Biotechnology

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Starting material from 1.5–3 mL cultures

Protocol tips
Starting material from 1.5–3 mL cultures

Publication protocol

Two methods were used for plasmid isolation: alkaline lysis according to Sambrook and Russel14 and a commercial kit – Plazmid Mini (A&A Biotechnology, Poland). As a control we used Escherichia coli V517 strain, which possesses eight cryptic plasmids
of different sizes.

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Papers

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Manufacturer protocol

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