Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Wash the cells twice with serum-free buffer or medium to remove serum esterase activity	Incubate the cells for 30-45 minutes at room temperature

Upstream tips
Wash the cells twice with serum-free buffer or medium to remove serum esterase activity
Protocol tips
Incubate the cells for 30-45 minutes at room temperature

Publication protocol

A co-culture model was conducted by culturing fibroblast cells and C. albicans together in a sterile 24-well plate, as adapted by [32]. First, oral fibroblast cells (ATCC: CRL2014) were seeded in Dulbecco’s Modified Eagle’s Medium (DMEM) with Fetal Bovine Serum (FBS) (Gibco) at 37°C in 5% CO2 for 24 hours. The medium was then replaced with an inoculum of 5x103 to 2.5x103 CFU/ml C. albicans (MYA2876) grown in DMEM without FBS. Fibroblast cells and C. albicans were treated with 62.5 μM of lichochalcone-A. The plate was then incubated at 37°C in 5% CO2 for 24 hours. The vehicle control tested was 1% ethanol and the positive control was fluconazole (32 μM). The distribution of dead and live fibroblast cells was examined using the viability/cytotoxicity assay kit for animal live & dead cells (Biotium), which contains a mixture of Calcein AM and EthDIII. Calcofluor white (Sigma) was used to stain C. albicans. Fluorescent images of the double staining were captured using fluorescence microscopy (EVOS fl microscope AMG, Bothell, WA, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay yeast - Candida albicans using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells from Biotium.

Manufacturer protocol

Download the product protocol from Biotium for Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Live / Dead assay yeast - Candida albicans using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells from Biotium. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.