EZ-10 Spin Column Plasmid DNA Miniprep Kit

Protocol tips

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	Starting material from 1.5–5 mL cultures

Protocol tips
Starting material from 1.5–5 mL cultures

Publication protocol

The ability of each E. coli colony to conjugate was assessed by transconjugant colony growth at each dilution the following day. One colony was selected, and the plasmid isolated from this strain was named pTA-Mob 2.0. Plasmid DNA was isolated using the EZ-10 Spin Column Plasmid DNA Miniprep Kit (BioBasic Inc., #BS413, Markham, ON, Canada) and sent for complete plasmid sequencing at the Massachusetts General Hospital DNA Core.

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Manufacturer protocol

Download the product protocol from Bio Basic for EZ-10 Spin Column Plasmid DNA Miniprep Kit below.

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