LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 1 µL of Component SYTO and 1 µL of Component propidium iodide. After stain is added, each tube should be vortexed gently. Incubate all samples at room temperature or 37ºC protected from light for 15–30 minutes

Protocol tips
Add 1 µL of Component SYTO and 1 µL of Component propidium iodide. After stain is added, each tube should be vortexed gently. Incubate all samples at room temperature or 37ºC protected from light for 15–30 minutes

Publication protocol

Urediniospore viability was determined by three methods: (i) the FungaLight yeast vitality kit (Molecular Probes Inc., Eugene, OR) containing CFDA, acetoxymethyl ester (AM) and PI; (ii) the LIVE/DEAD yeast viability kit (Molecular Probes Inc.) containing FUN 1 and calcofluor white M2R; and (iii) an in vitro germination test. Three spore samples (live, heat-killed, and a 1:1 mix) were tested for each method.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry below.

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