LIVE/DEAD™ Yeast Viability Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 1 µL of Component A (FUN® 1 cell stain) and 5 µL of Component B (Calcofluor® White M2R) to 1 mL of yeast suspension (10 µM and 25 µM final concentrations of each of the dyes, respectively). Incubate at 30°C in the dark for 30 minutes

Protocol tips
Add 1 µL of Component A (FUN® 1 cell stain) and 5 µL of Component B (Calcofluor® White M2R) to 1 mL of yeast suspension (10 µM and 25 µM final concentrations of each of the dyes, respectively). Incubate at 30°C in the dark for 30 minutes

Publication protocol

Urediniospore viability was determined by three methods: (i) the FungaLight yeast vitality kit (Molecular Probes Inc., Eugene, OR) containing CFDA, acetoxymethyl ester (AM) and PI; (ii) the LIVE/DEAD yeast viability kit (Molecular Probes Inc.) containing FUN 1 and calcofluor white M2R; and (iii) an in vitro germination test. Three spore samples (live, heat-killed, and a 1:1 mix) were tested for each method.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Yeast Viability Kit below.

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