E.Z.N.A.® Plasmid Mini Kit I, (Q-spin)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	starting material 1-5 mL bacterial cultures

Protocol tips
starting material 1-5 mL bacterial cultures

Publication protocol

Extraction of prophage DNA from bacterial cells was performed using a plasmid extraction kit (D6943; Omega Bio-Tek), as per the manufacturer's instruction. Since XH001 is Gram positive, mechanical cell breakage using bead-beating for 10 s was used to increase the cell lysis efficiency and DNA yield. The DNA isolated from 1 ml of bacterial culture (OD600, 1.0) was about 36 ng/μl, with a final volume of 40 μl. However, the bead-beating tends to break bacterial genomic DNA, which likely increased the host DNA contamination when extracting prophage genome using a plasmid extraction kit

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Plasmid Isolation Actinomyces odontolyticus using E.Z.N.A.® Plasmid Mini Kit I, (Q-spin) from Omega Bio Tek.

Manufacturer protocol

Download the product protocol from Omega Bio Tek for E.Z.N.A.® Plasmid Mini Kit I, (Q-spin) below.

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Videos

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