Cytochrome C Monoclonal Antibody (37BA11)

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	-Mouse (1:1000)

Protocol tips
-Mouse (1:1000)

Publication protocol

IB analyses were performed using standard SDS–PAGE chemiluminescent procedures and antibodies used were as follows: Rabbit polyclonal to Tmem65 antibody (HPA025020, Sigma; 1:500 dilution for IB; 1:1,000 dilution for IF), rabbit polyclonal to Tmem65 antibody (ab107871, Abcam; 1:400 dilution for IB), Tmem65 (sc-87464, Santa Cruz; 1:400 dilution for IB), rabbit polyclonal to the Na+/Ca+ exchanger (32881, Santa Cruz; 1:200 dilution for IB), mouse monoclonal Na+/K+ ATPase (a6F, Developmental Studies Hybridoma Bank; 1:500 dilution for IB), rabbit ubiquitin carboxyl-terminal esterase L1 (U5383, Sigma; 1:2,000 dilution for IB), chicken polyclonal glyceraldehyde-3-phosphate dehydrogenase (Ab14247, Abcam; 1:1,000 dilution for IB), calreticulin (Ab2907, Abcam; 1:1,000 dilution for IB), mouse monoclonal cytochrome c (456100, Invitrogen; 1:1,000 dilution for IB), mouse monoclonal transportin-1 (ab10303, Abcam; 1:1,000 dilution for IB), mouse dysferlin (PA1-20910, Affinity Bioreagents; 1:200 dilution for IB), rabbit polyclonal glypican-1 (ab55971, Abcam; 1:500 dilution for IB), mouse dihydropyridine receptor alpha-2 (MA3-921, Affinity Bioreagents; 1:500 dilution for IB), mouse cardiomyopathy-associated 1 (611524, BD Transduction Laboratories; 1:1,000 dilution for IB), dihydropyrimidinase-like 3 protein (AB5454, Millipore; 1:2,500 dilution for IB), ryanodine receptor 2 (34C, Life Technologies; 1:2,500 dilution for IB), histidine tag (6xHis, AB18184, Abcam; 1:1,000 dilution for IB), Cx43 (AB66151, Abcam; 1:500 dilution for IB, 1:50 for IF), desmoplakin (AB16434, Abcam; 1:200 dilution for IB, 1:100 for IF), NCAD (AB18203, Abcam; 1:200 dilution for IB, 1:100 for IF), alpha-tubulin (2144, Cell Signaling; 1:1,000 for IB), actin (4967, Cell Signaling; 1:2,000 for IB), plasma membrane Ca2+ ATPase (MA3-914, Thermo Scientific; 1:1,000 for IB) and alpha actinin (MA1-22863, Fisher; 1:400 for IF). Secondary antibodies of anti-mouse IgG horse radish peroxidase (HRP) conjugate (W402B, Promega; 1:2,500 dilution) and anti-rabbit IgG HRP (W401B, Promega; 1:2,500 dilution) were used.

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