Anti-EGFR (phospho Y1068) antibody (ab5644)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

Immunoblot analysis was performed as previously described16. Immunoblotting was performed with the following antibodies: anti-pan-RAS (sc-4), anti-β-catenin (sc-7199), anti-ERK (sc-514302), anti-APC (sc-896), anti-PCNA (sc-56), anti-KRAS (sc-30), and anti-Myc (sc-40) purchased from Santa Cruz Biotechnology; anti-p-ERK (9101) and anti-α-tubulin (3873) obtained from Cell Signaling Technology; and anti-EGFR (ab52894) and anti-p-EGFR (ab5644) purchased from Abcam (Cambridge, United Kingdom).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting EGFR using Anti-EGFR (phospho Y1068) antibody (ab5644) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-EGFR (phospho Y1068) antibody (ab5644) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting EGFR using Anti-EGFR (phospho Y1068) antibody (ab5644) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.