β-Actin Antibody #4967

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

The following antibodies were used: mouse monoclonal anti-FLAG M2 (Sigma-Aldrich), mouse monoclonal anti-ubiquitinated protein (FK2; Nippon Bio-Test Laboratories), mouse monoclonal anti-Rab30 (Abcam, ab156774), rabbit monoclonal anti-Atg5 (Cell Signaling Technology, #4967), rabbit monoclonal anti-β-actin (Cell Signaling Technology, #4970), mouse monoclonal anti-LAMP1 (Santa Cruz, sc-20011), and rabbit monoclonal anti-GM130 (BD Transduction Laboratories, 610822). The secondary antibodies used for immunoblotting were horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Jackson Immunoresearch Laboratories). The fluorescent secondary antibodies used for immunofluorescence were Alexa Fluor 488-conjugated goat anti-mouse or anti-rabbit IgG, Alexa Fluor 594-conjugated goat anti-mouse or anti-rabbit IgG, or Alexa Fluor 660-conjugated goat anti-mouse or anti-rabbit IgG (Molecular Probes/Invitrogen).

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for β-Actin Antibody #4967 below.

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