Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 0.7 mL isopropyl alcohol into the tube with 0.3 mL of 0.85% NaCl and mix well (final concentration of isopropyl alcohol: 70%) for preparing dead bacteria. Incubate both samples at room temperature for 1 hour, mixing every 15 minutes

Protocol tips
Add 0.7 mL isopropyl alcohol into the tube with 0.3 mL of 0.85% NaCl and mix well (final concentration of isopropyl alcohol: 70%) for preparing dead bacteria. Incubate both samples at room temperature for 1 hour, mixing every 15 minutes

Publication protocol

The heat-inactivated C. sporogenes bacteria were subjected to a bacterial viability test to confirm the effectiveness of the heat-inactivation process. The Viability/Cytotoxicity Assay kit for Bacterial Live & Dead Cells (Biotium, USA), was used to stain the IB using fluorescent nucleic acid dyes following the manufacturer’s protocol. DMAO is a green dye that stains both live and dead bacteria while Ethidium Homodimer-III (EthD-III) is a red dye that only stains dead bacteria. Images were obtained using a fluorescence microscope (Olympus, Japan)

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Manufacturer protocol

Download the product protocol from Biotium for Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells below.

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