TNF-α Antibody #3707

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

5 × 106 JURKAT cells or 2 × 106 U2OS, A549, HEK293 cells were seeded in 10 cm plates. After the respective treatments cells were washed two times with phosphate-buffered saline (PBS) and lysed with RIPA buffer containing a cocktail of protease inhibitor (P8340 Sigma), inhibitors of phosphatases (P0044 and P5726 Sigma) and PMSF 1 mM. Protein quantification was performed using BCA kit (Pierce). After the electrophoresis, the proteins were transferred to a nitrocellulose membrane (midi kit, Bio-Rad) with Trans-Blot Turbo Transfer System (Bio-Rad). Finally, membranes were imaged using the ChemiDoc MP imager (Bio-Rad).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting TNF-alpha using TNF-α Antibody #3707 from Cell Signaling Technology.

Manufacturer protocol

Download the product protocol from Cell Signaling Technology for TNF-α Antibody #3707 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting TNF-alpha using TNF-α Antibody #3707 from Cell Signaling Technology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.