Estrogen Receptor alpha Monoclonal Antibody (SP1)

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	-Rabbit (1:10)

Protocol tips
-Rabbit (1:10)

Publication protocol

Primary antibodies and fold dilutions against GAPDH (1:20, goat polyclonal antibody (pAb); SAB2500450, Sigma), β-tubulin (1:10, rabbit pAb; ab6046, Abcam), EpCAM (1:10, rabbit pAb; 3599, Cell Signaling), EGFR (1:10, mouse monoclonal antibody; 2322, Cell Signaling), ER (1:10, rabbit monoclonal antibody; RM-9101-S0, ThermoScientific), HER2 (1:10, mouse monoclonal antibody; MA513105, Pierce), ERK1/2 (1:10, rabbit monoclonal antibody; 4695, Cell Signaling), eIF4E (1:10, rabbit monoclonal antibody; 2067, Cell Signaling), mTOR (1:10, rabbit monoclonal antibody; 2983, Cell Signaling), panCK (1:10, rabbit pAb; Z0622, Dako) and CK8 (1:10, mouse monoclonal antibody; C5301, Sigma) were the immunoprobes in both breast cancer cell lines (BT-20, MCF7 and SK-BR-3) and patient-derived CTCs. For analysis of the MCF7-GFP cell line, an anti-GFP antibody (ab6673, Abcam) followed by anti-goat AlexaFluor 555-conjugated secondary antibody (A21432, Invitrogen) were used. Secondary antibodies to goat IgG pre-labelled with AlexaFluor 488 and 555 (A11055 and A21432), mouse IgG pre-labelled with AlexaFluor 488, 555 and 647 (A21202, A31570 and A31571), and rabbit IgG pre-labelled with AlexaFluor 488, 555 and 647 (A21206, A31572 and A31573) were used as prepared by the vendor (Invitrogen). All secondary antibodies were applied as a 1:20 dilution.

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Manufacturer protocol

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