miRNeasy Mini kit

RNA isolation / purification Cells - primary hematopoietic stem cells

Experiment
RNA isolation / purification Cells - primary hematopoietic stem cells
Product
miRNeasy Mini kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 1x10^6 cells

Publication protocol

Qiagen's miRNeasy isolation kit (Valancia, CA, USA) was used to isolate total RNA. As a first step, tissue was placed in Qiazol and passed through a 1-mL syringe attached to a 17-G needle several times. RNA samples were run on an Agilent BioAnalyzer microfluidics chip RNA Nano 6000 (Agilent Technologies, Santa Clara, CA, USA) to assess quality and quantity. Of four samples, the three samples having the highest quality (RNA Integrity Number [RIN] ≥ 7 value) were labeled as probes for the Affymetrix GeneChip microarrays (Affymetrix, Santa Clara, CA, USA).

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Reviews

miRNeasy Mini kit from Qiagen has not yet been reviewed for this experiment

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary hematopoietic stem cells using miRNeasy Mini kit from Qiagen.

Paper title
Intravitreal Administration of Human Bone Marrow CD34+ Stem Cells in a Murine Model of Retinal Degeneration.
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary hematopoietic stem cells using miRNeasy Mini kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

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