miRNeasy Mini kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from up to 1x10^6 cells

Upstream tips
- This kit can be used for the isolation of RNA from up to 1x10^6 cells

Publication protocol

Qiagen's miRNeasy isolation kit (Valancia, CA, USA) was used to isolate total RNA. As a first step, tissue was placed in Qiazol and passed through a 1-mL syringe attached to a 17-G needle several times. RNA samples were run on an Agilent BioAnalyzer microfluidics chip RNA Nano 6000 (Agilent Technologies, Santa Clara, CA, USA) to assess quality and quantity. Of four samples, the three samples having the highest quality (RNA Integrity Number [RIN] ≥ 7 value) were labeled as probes for the Affymetrix GeneChip microarrays (Affymetrix, Santa Clara, CA, USA).

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

Download manufacturer protocol

Videos

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