p-JNK Antibody (G-7): sc-6254

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:500) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Protocol tips
-Mouse (1:500) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Publication protocol

The proteins of the hippocampus were homogenized in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz, Dallas, TX, USA) with phosphatase inhibitor (Boster, Wuhan, China) and protease inhibitor (Beyotime, Shanghai, China). We examined the expression of α7nAChR, TNF-α, IL-1β, CGRP, and p-JNK using Western blotting as previously described.55 The primary antibodies were α7nAChR (1:200, sc-58607), TNF-α (1:500, sc-52B83), IL-1β (1:500, sc-12742), CGRP (1:500, sc-57053), p-JNK (1:500, sc-6254), and β-actin (1:4000, 20536-1-AP). The immunoreacted bands were visualized with a BeyoECL Plus Kit (P1008; Beyotime) and quantified with an imaging system (Fusion, Munich, Germany).

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Manufacturer protocol

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