E-cadherin monoclonal antibody (ECCD-2)

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	-Rat (1:1000)

Protocol tips
-Rat (1:1000)

Publication protocol

After removal of the perineurium, SNs were homogenized in microcentrifugation tubes (Eppendorf) using chilled pestles (Argos Technologies) in lysis buffer (2% SDS, 10 mM NaCl, 25 mM Tris-HCl pH 7.4, proteinase inhibitor cocktail (Sigma), phosphatase inhibitor (Roche)). Lysates were supplemented with sample buffer (6.2 mM Tris, 1% β-mercaptoethanol, 2% glycerol) and further processed using standard SDS-PAGE and western blot methods. The following antibodies were used: Notch1 (MAB5352, Millipore, 1:1,000), HES1 (ab71559, Abcam, 1:1,000), EGR2 (13491-1-AP, Proteintech, 1:1,000), E-cadherin (ALX-804-202-C100, Enzo, 1:1,000), HMGA2 (#8179, Cell Signalling, 1:1,000), β-actin (A5316, Sigma, 1:5,000), MBP (MCA409s, Serotec, 1:1,000). Secondary antibodies were obtained from Jackson and Promega. Signal detection was carried out using Fusion FX7 (Vilber Lourmat) and band intensities were quantified using Quantity One software (Biorad).

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