beta Catenin Monoclonal Antibody (CAT-5H10)

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	-Mouse (1:500)

Protocol tips
-Mouse (1:500)

Publication protocol

To evaluate SOX12 and β‐catenin protein expression, proteins extracted from the THP1 cell line were separated by a 10% sodium dodecyl sulphate‐polyacrylamide gel electrophoresis, transferred to a nitrocellulose membrane and blotted with mouse polyclonal anti‐SOX12 (1:1000) (4A7, Sigma), mouse monoclonal anti‐total β‐catenin (1:500) (13‐8400, Invitrogen) or mouse monoclonal anti‐β‐actin (1:10000) (A1978, Sigma) antibodies. The membranes were visualized using the Chemiluminescent Substrate method with the SuperSignal West Pico kit (Pierce Biotechnology, Inc., Rockford, IL, USA) and developed using X‐ray film.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for beta Catenin Monoclonal Antibody (CAT-5H10) below.

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