Anti-N-Cadherin Antibody, clone 13A9

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:5000)

Protocol tips
-Mouse (1:5000)

Publication protocol

ADAM10 was detected using a rabbit monoclonal antibody (GeneTex, EPR5622, 1:3,000). Human and murine PLEKHA7 was detected with previously described (28) rabbit polyclonal antibody against recombinant C-terminal fragments of PLEKHA7 (1:2,000 Western blot and 1:200 immunofluorescence). Canine PLEKHA7 was detected by Western blot with a polyclonal guinea pig antibody against recombinant C-terminal fragments of canine PLEKHA7 used at 1:1,000 (antibody a kind gift from Sandra Citi, University of Geneva, Geneva, Switzerland). Afadin was detected using a rabbit polyclonal antibody (Sigma, A0349, 1:2,000). N-cadherin was detected using a mouse monoclonal antibody (EMD Millipore, clone 13A9, 1;5,000). α-catenin was detected using a rabbit polyclonal antibody kindly provided by W. James Nelson, Stanford University, Stanford, CA, at 1:5,000. p38 MAPK was visualized with rabbit polyclonal antibody (Cell Signaling Technology, 9212, 1:1,000). Phosphorylated p38 MAPK (Thr180/Tyr182) was visualized using a rabbit polyclonal antibody (Cell Signaling Technology, 9211, 1:1,000). GAPDH was visualized with rabbit polyclonal antibody (Cell Signaling Technology, 5174, 1:5,000). S. aureus α-toxin was visualized with a rabbit antibody against H35L mutant α-toxin provided by Fabio Bagnoli at 1:1,000. Expression of FLAG-tagged PLEKHA7 constructs were visualized using rabbit polyclonal antibody anti-DYKDDDK (Cell Signaling Technology, 2368s, 1:2,500).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting N‐cadherin using Anti-N-Cadherin Antibody, clone 13A9 from Merck Millipore.

Manufacturer protocol

Download the product protocol from Merck Millipore for Anti-N-Cadherin Antibody, clone 13A9 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting N‐cadherin using Anti-N-Cadherin Antibody, clone 13A9 from Merck Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.