Akt1 Antibody (B-1): sc-5298

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Protocol tips
-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Publication protocol

Antibodies for phospho-Akt (9271, 1:1000), Phospho-Stat5 (9359, 1:1000), Stat5 (9363, 1:1000), α-Tubulin (2144,1:2000), Phospho-Ulk1 Ser555 (5869, 1:500), Phospho-Ulk1 Ser467 (4634, 1:1000), Phospho-Ulk1 Ser757 (14202, 1:1000), Ulk1 (8054, 1:1000), Phospho-AMPKα (2535, 1:1000), AMPKα (5831, 1:1000), Phospho-p70 S6K (9206, 1:1000), p70 S6K (9202, 1:1000), FIP200 (12436, 1:1000), mTOR (2972, 1:1000), and Tbkbp1 (8605, 1:1000) were from Cell Signaling Technology. Antibody for Akt1(sc-5298,1:1000), c-Myc(sc-40, 1:1000), Ankrd28(sc-393032,1:1000) and Hsp60(sc-13115, 1:2000) was from Santa Cruz Biotechnology. Antibody for PPP6C (A300-844A, 1:1000) was obtained from Bethyl Laboratories, Inc. Horseradish peroxidase–conjugated anti-HA antibody (3F10, 1:2000) was purchased from Roche. Anti-LC3 antibody (L7543, 1:1000) and horseradish peroxidase–conjugated anti-Flag antibody (M2, 1:5000) were purchased from Sigma-Aldrich. Horseradish peroxidase–conjugated anti-c-Myc (MCA2200P, 1:1000) was from Bio-Rad. Horseradish Peroxidase-conjugated Donkey anti-mouse IgG (715-035-151, 1:10,000), Horseradish Peroxidase-conjugated Goat anti-mouse IgG light chain (115-035-174, 1:2000), Horseradish Peroxidase-conjugated Donkey anti-rabbit IgG (711-035-152, 1:10,000), and Horseradish Peroxidase-conjugated Mouse anti-rabbit IgG light chain (211-032-171, 1:2000) were purchased from Jackson ImmunoResearch.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for Akt1 Antibody (B-1): sc-5298 below.

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